Mufti N, Kong Y, Cirillo JD, Maitland KC, “Fiber optic microendoscopy for preclinical study of bacterial infection dynamics,” Biomedical Optics Express, Vol. 2, No. 5, 1121-1134, 2011.
We explore the use of fiber optic microendoscopy to image and quantify bacterial infection in the skin and lungs using an animal model. The contact probe fiber bundle fluorescence microendoscope has a 4 µm resolution, a 750 µm field of view, and a 1 mm outer diameter. Subcutaneous and intra-tracheal infections of fluorescent Mycobacterium bovis Bacillus Calmette-Guérin (BCG) bacteria were detected in situ from inocula down to 104 and 107 colony forming units, respectively.